6chv

X-ray diffraction
2.9Å resolution

Proteus vulgaris HigA antitoxin bound to DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Antitoxin HigA Chains: A, B, C, D, G, H
Molecule details ›
Chains: A, B, C, D, G, H
Length: 121 amino acids
Theoretical weight: 13.21 KDa
Source organism: Proteus vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7A224 (Residues: 1-104; Coverage: 100%)
Gene name: higA
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
pHigCryst3 Chains: E, I, K
Molecule details ›
Chains: E, I, K
Length: 21 nucleotides
Theoretical weight: 6.39 KDa
Source organism: Proteus vulgaris
Expression system: Not provided
pHigCryst4 Chains: F, J, L
Molecule details ›
Chains: F, J, L
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
Source organism: Proteus vulgaris
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P65
Unit cell:
a: 79.449Å b: 79.449Å c: 399.427Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.225 0.26
Expression systems:
  • Escherichia coli
  • Not provided