› 6ce5

Solution NMR

NMR structure of the Rous sarcoma virus matrix protein (M-domain) in the presence of myo-inositol hexakisphosphate

Released:
DOI: 10.2210/pdb6ce5/pdb
PDB entry 6ce5 coloured by chain, ensemble of 20 models, front view.
PDB entry 6ce5 coloured by chain, ensemble of 20 models, side view.
PDB entry 6ce5 coloured by chain, ensemble of 20 models, top view.
Source organism: Rous sarcoma virus - Prague C
Primary publication:
Structural basis for targeting avian sarcoma virus Gag polyprotein to the plasma membrane for virus assembly.
Vlach J, Eastep GN, Ghanam RH, Watanabe SM, Carter CA, Saad JS
J Biol Chem 293 18828-18840 (2018)
PMID: 30309983

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136816 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrix protein p19 Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.21 KDa
Source organism: Rous sarcoma virus - Prague C
Expression system: Escherichia coli
UniProt:
  • Canonical: P03354 (Residues: 1-87; Coverage: 5%)
Gene name: gag-pol
Sequence domains: Retroviral M domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 96%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Interplay between HIV-1 Gag Binding to the Plasma Membrane and Env Incorporation.
Murphy et al. (2020)
1 more

1 mention without citation

Structural basis for targeting avian sarcoma virus Gag polyprotein to the plasma membrane for virus assembly.
Vlach et al. (2018)