6cdz

X-ray diffraction
2.4Å resolution

E. coli thymidylate synthase mutant I264Am

Released:
Source organism: Escherichia coli K-12
Entry authors: Finer-moore JS, Lee TT, Stroud RM

Function and Biology Details

Reaction catalysed:
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 30.45 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A884 (Residues: 1-263; Coverage: 100%)
Gene names: JW2795, b2827, thyA
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 67.658Å b: 80.451Å c: 94.08Å
α: 90° β: 103.5° γ: 90°
R-values:
R R work R free
0.183 0.18 0.229
Expression system: Escherichia coli