› 6cda

X-ray diffraction
2Å resolution

Crystal structure of L34A CzrA in the Zn(II)bound state

Released:
DOI: 10.2210/pdb6cda/pdb
PDB entry 6cda coloured by chain, front view.
PDB entry 6cda coloured by chain, side view.
PDB entry 6cda coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Functional Role of Solvent Entropy and Conformational Entropy of Metal Binding in a Dynamically Driven Allosteric System.
Capdevila DA, Edmonds KA, Campanello GC, Wu H, Gonzalez-Gutierrez G, Giedroc DP
J Am Chem Soc 140 9108-9119 (2018)
PMID: 29953213

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH arsR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.97 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: O85142 (Residues: 1-106; Coverage: 100%)
Gene names: A6760_11845, BN1321_350009, CV021_10345, CzrA, EP54_06885, EQ90_13065, FAF17_13815, G6W63_05680, G6Y24_05035, GO793_16165, GO814_05535, GO941_16585, GO942_06145, GQX37_08730, GZ156_05085, GZ163_08250, H2639_10805, HMPREF3211_00009, M1K003_2263, NCTC10702_03381, NCTC13131_01480, NCTC5664_01801, NCTC6133_02909, NCTC7878_02904, NCTC7972_02574, QU38_06130, SAMEA2078260_01939, SAMEA2078588_01910, SAMEA2080344_01776, SAMEA2081063_01791, SAMEA4008575_00680, SAMEA70146418_02191, SAMEA70153168_02326, SAMEA70245418_02324, czrA, czrA_1, czrA_2, rzcA
Sequence domains: Bacterial regulatory protein, arsR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 2 x CL
Ligand PG4 1 x PG4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P41212
Unit cell:
a: 31.987Å b: 31.987Å c: 183.392Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.242
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The requirement for cobalt in vitamin B12: A paradigm for protein metalation.
Osman et al. (2021)
2 more

1 mention without citation

Structural and functional mapping of ars gene cluster in Deinococcus indicus DR1.
Ranganathan et al. (2023)