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X-ray structure of GenB1 from micromonospora echinospora in complex with neamine and PLP (as the external aldimine)

X-ray diffraction
1.6Å resolution
PDB entry 6cbo coloured by chain, front view.
PDB entry 6cbo coloured by chain, side view.
PDB entry 6cbo coloured by chain, top view.
Source organism: Micromonospora echinospora
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: C-6' aminotransferase
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Multimeric state: homo dimer
Accessible surface area: 29064.14 Å2
Buried surface area: 8305.29 Å2
Dissociation area: 3,873.04 Å2
Dissociation energy (ΔGdiss): 41.34 kcal/mol
Dissociation entropy (TΔSdiss): 14.89 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-180690
Assembly 2 (preferred)
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Assembly Name: C-6' aminotransferase
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Multimeric state: homo dimer
Accessible surface area: 29090.21 Å2
Buried surface area: 8347.3 Å2
Dissociation area: 3,738.99 Å2
Dissociation energy (ΔGdiss): 48.08 kcal/mol
Dissociation entropy (TΔSdiss): 14.86 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-180690

Macromolecules

C-6' aminotransferase
Chains: A, B, C, D
Length: 439 amino acids
Theoretical weight: 47.47 KDa
Source organism: Micromonospora echinospora
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70KD9 (Residues: 1-417; Coverage: 100%)
Gene names: gacK, genB1, gntW
Pfam: Aminotransferase class-III
InterPro:
Putative glutamate-1-semialdehyde aminotransferase in PDB entry 6cbo, assembly 2, front view.
Putative glutamate-1-semialdehyde aminotransferase in PDB entry 6cbo, assembly 2, side view.
Putative glutamate-1-semialdehyde aminotransferase in PDB entry 6cbo, assembly 2, top view.
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