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THE CRYSTAL STRUCTURE OF THE W169Y MUTANT OF ALPHA-GLUCOSIDASE (GH 31) FROM RUMINOCOCCUS OBEUM ATCC 29174 in complex with miglitol

X-ray diffraction
1.743Å resolution
PDB entry 6ca3 coloured by chain, front view.
PDB entry 6ca3 coloured by chain, side view.
PDB entry 6ca3 coloured by chain, top view.
Source organism: Blautia obeum ATCC 29174
Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Glycosyl hydrolase, family 31
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Multimeric state: monomeric
Accessible surface area: 24500.87 Å2
Buried surface area: 165.03 Å2
Dissociation area: 82.52 Å2
Dissociation energy (ΔGdiss): -1.02 kcal/mol
Dissociation entropy (TΔSdiss): 0.79 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-107828
Assembly 2
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Assembly Name: Glycosyl hydrolase, family 31
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Multimeric state: monomeric
Accessible surface area: 24413.24 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-107828
Assembly 3
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Assembly Name: Glycosyl hydrolase, family 31
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Multimeric state: homo dimer
Accessible surface area: 43742.33 Å2
Buried surface area: 5336.8 Å2
Dissociation area: 2,551.07 Å2
Dissociation energy (ΔGdiss): 19.55 kcal/mol
Dissociation entropy (TΔSdiss): 15.68 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-107829

Macromolecules

Glycosyl hydrolase, family 31
Chains: A, B
Length: 666 amino acids
Theoretical weight: 77.43 KDa
Source organism: Blautia obeum ATCC 29174
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5ZY13 (Residues: 1-663; Coverage: 100%)
Gene name: RUMOBE_03919
Pfam:
InterPro:
CATH:
Glycoside hydrolase family 31 N-terminal domain-containing protein in PDB entry 6ca3, assembly 1, front view.
Glycoside hydrolase family 31 N-terminal domain-containing protein in PDB entry 6ca3, assembly 1, side view.
Glycoside hydrolase family 31 N-terminal domain-containing protein in PDB entry 6ca3, assembly 1, top view.
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