6ca3

X-ray diffraction
1.74Å resolution

THE CRYSTAL STRUCTURE OF THE W169Y MUTANT OF ALPHA-GLUCOSIDASE (GH 31) FROM RUMINOCOCCUS OBEUM ATCC 29174 in complex with miglitol

Released:
Source organism: Blautia obeum ATCC 29174
Entry authors: Tan K, Tesar C, Jedrzejczak R, Joachimiak A, Midwest Center for Macromolecular Research (MCMR)

Function and Biology Details

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gal_mutarotas_2 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 666 amino acids
Theoretical weight: 77.43 KDa
Source organism: Blautia obeum ATCC 29174
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5ZY13 (Residues: 1-663; Coverage: 100%)
Gene name: RUMOBE_03919
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 64.685Å b: 70.826Å c: 88.103Å
α: 111.32° β: 107.42° γ: 97.66°
R-values:
R R work R free
0.155 0.153 0.191
Expression system: Escherichia coli BL21(DE3)