› 6c9z

X-ray diffraction
2.1Å resolution

THE CRYSTAL STRUCTURE OF THE alpha-Glucosidase (GH 31) W169Y mutant FROM RUMINOCOCCUS OBEUM ATCC 29174 in complex with voglibose

Released:
DOI: 10.2210/pdb6c9z/pdb
PDB 6c9z coloured by chain and viewed from the front.
PDB 6c9z coloured by chain and viewed from the side.
PDB 6c9z coloured by chain and viewed from the top.
Source organism: Blautia obeum ATCC 29174
Entry authors: Tan K, Tesar C, Jedrzejczak R, Joachimiak A, Midwest Center for Macromolecular Research (MCMR)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gal_mutarotas_2 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 666 amino acids
Theoretical weight: 77.43 KDa
Source organism: Blautia obeum ATCC 29174
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5ZY13 (Residues: 1-663; Coverage: 100%)
Gene name: RUMOBE_03919
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand VOG 2 x VOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 67.751Å b: 126.346Å c: 88.191Å
α: 90° β: 107.82° γ: 90°
R-values:
R R work R free
0.194 0.192 0.246
Expression system: Escherichia coli BL21(DE3)

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