PDBe 6c23

Electron Microscopy
3.9Å resolution

Cryo-EM structure of PRC2 bound to cofactors AEBP2 and JARID2 in the Compact Active State

Released:
Source organism: Homo sapiens
Related structures: EMD-7334

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Polycomb protein SUZ12 Chains: A, M, Q
Molecule details ›
Chains: A, M, Q
Length: 739 amino acids
Theoretical weight: 83.18 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q15022 (Residues: 1-739; Coverage: 100%)
Gene names: CHET9, JJAZ1, KIAA0160, SUZ12
Sequence domains: VEFS-Box of polycomb protein
Protein Jumonji Chain: E
Molecule details ›
Chain: E
Length: 348 amino acids
Theoretical weight: 37.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q92833 (Residues: 106-450; Coverage: 28%)
Gene names: JARID2, JMJ
Histone-lysine N-methyltransferase EZH2 Chains: C, K
Molecule details ›
Chains: C, K
Length: 746 amino acids
Theoretical weight: 85.49 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q15910 (Residues: 1-746; Coverage: 100%)
Gene names: EZH2, KMT6
Sequence domains:
Polycomb protein EED Chain: L
Molecule details ›
Chain: L
Length: 441 amino acids
Theoretical weight: 50.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O75530 (Residues: 1-441; Coverage: 100%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Histone-binding protein RBBP4 Chain: N
Molecule details ›
Chain: N
Length: 425 amino acids
Theoretical weight: 47.71 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q09028 (Residues: 1-425; Coverage: 100%)
Gene names: RBAP48, RBBP4
Sequence domains:
JARID2-substrate Chain: O
Molecule details ›
Chain: O
Length: 7 amino acids
Theoretical weight: 736 Da
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Zinc finger protein AEBP2 Chain: P
Molecule details ›
Chain: P
Length: 295 amino acids
Theoretical weight: 33.01 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6ZN18 (Residues: 209-503; Coverage: 57%)
Gene name: AEBP2
SUZ12 Chain: Z
Molecule details ›
Chain: Z
Length: 135 amino acids
Theoretical weight: 11.51 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Protein Jumonji Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.71 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q92833 (Residues: 106-450; Coverage: 28%)
Gene names: JARID2, JMJ

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-7334
Expression system: Trichoplusia ni