› 6c0t

X-ray diffraction
1.98Å resolution

Crystal structure of cGMP-dependent protein kinase Ialpha (PKG Ialpha) catalytic domain bound with N46

Released:
DOI: 10.2210/pdb6c0t/pdb
PDB entry 6c0t coloured by chain, front view.
PDB entry 6c0t coloured by chain, side view.
PDB entry 6c0t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for selective inhibition of human PKG Iα by the balanol-like compound N46.
Qin L, Sankaran B, Aminzai S, Casteel DE, Kim C
J Biol Chem 293 10985-10992 (2018)
PMID: 29769318

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 39.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q13976 (Residues: 327-671; Coverage: 51%)
Gene names: PRKG1, PRKG1B, PRKGR1A, PRKGR1B
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand EE4 1 x EE4
Ligand DMS 1 x DMS
Ligand PGE 1 x PGE
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P42
Unit cell:
a: 85.73Å b: 85.73Å c: 50.664Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.244
Expression system: Trichoplusia ni

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Citations

2 citation in other articles

Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates.
Pérez-López et al. (2020)
1 more

7 mentions without citation

Recent Advances in Pain Management: Relevant Protein Kinases and Their Inhibitors.
Giraud et al. (2021)
6 more