Structure analysis

Thermus thermophilus 70S containing 16S G299A point mutation and near-cognate ASL Leucine in A site.

X-ray diffraction
3.68Å resolution
Source organism: Thermus thermophilus HB8
Assembly composition:
hetero 56-mer (preferred)
Entry contents: 49 distinct polypeptide molecules
6 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 56-mer
Accessible surface area: 786299.82 Å2
Buried surface area: 278036.7 Å2
Dissociation area: 578.1 Å2
Dissociation energy (ΔGdiss): -5.24 kcal/mol
Dissociation entropy (TΔSdiss): 10.72 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143760
Assembly 2
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Multimeric state: hetero 56-mer
Accessible surface area: 784169.17 Å2
Buried surface area: 278695.78 Å2
Dissociation area: 574.09 Å2
Dissociation energy (ΔGdiss): -2.6 kcal/mol
Dissociation entropy (TΔSdiss): 10.73 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143760

Macromolecules

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Chains: QH, XH
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Pfam: Ribosomal protein S8
InterPro:

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Chains: QJ, XJ
Length: 105 amino acids
Theoretical weight: 11.95 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN7 (Residues: 1-105; Coverage: 100%)
Gene names: TTHA1693, rpsJ
Pfam: Ribosomal protein S10p/S20e
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Chains: QK, XK
Length: 129 amino acids
Theoretical weight: 13.74 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80376 (Residues: 1-129; Coverage: 100%)
Gene names: TTHA1666, rps11, rpsK
Pfam: Ribosomal protein S11
InterPro:

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Chains: QL, XL
Length: 132 amino acids
Theoretical weight: 14.64 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
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Chains: QO, XO
Length: 89 amino acids
Theoretical weight: 10.58 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:

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Chains: QP, XP
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:

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Chains: QQ, XQ
Length: 105 amino acids
Theoretical weight: 12.33 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY7 (Residues: 1-105; Coverage: 100%)
Gene names: TTHA1683, rpsQ
Pfam: Ribosomal protein S17
InterPro:

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Chains: QR, XR
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
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Chains: QS, XS
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP2 (Residues: 1-93; Coverage: 100%)
Gene names: TTHA1688, rpsS
Pfam: Ribosomal protein S19
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Chains: QT, XT
Length: 106 amino acids
Theoretical weight: 11.74 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
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Chains: QU, XU
Length: 27 amino acids
Theoretical weight: 3.35 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SIH3 (Residues: 1-27; Coverage: 100%)
Gene names: TTHA1396, rpsU
Pfam: 30S ribosomal protein Thx
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Chains: RF, YF
Length: 210 amino acids
Theoretical weight: 23.27 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN9 (Residues: 1-210; Coverage: 100%)
Gene names: TTHA1691, rplD
Pfam: Ribosomal protein L4/L1 family
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Chains: RN, YN
Length: 140 amino acids
Theoretical weight: 15.93 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60488 (Residues: 1-140; Coverage: 100%)
Gene names: TTHA1465, rplM
Pfam: Ribosomal protein L13
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Chains: RO, YO
Length: 122 amino acids
Theoretical weight: 13.32 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP8 (Residues: 1-122; Coverage: 100%)
Gene names: TTHA1682, rplN
Pfam: Ribosomal protein L14p/L23e
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Chains: RQ, YQ
Length: 141 amino acids
Theoretical weight: 15.99 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60489 (Residues: 1-141; Coverage: 100%)
Gene names: TTHA1685, rplP
Pfam: Ribosomal protein L16p/L10e
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Chains: RR, YR
Length: 118 amino acids
Theoretical weight: 13.75 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q9Z9H5 (Residues: 1-118; Coverage: 100%)
Gene names: TTHA1663, rpl17, rplQ
Pfam: Ribosomal protein L17
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Chains: RS, YS
Length: 112 amino acids
Theoretical weight: 12.64 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHQ4 (Residues: 1-112; Coverage: 100%)
Gene names: TTHA1676, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
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Chains: RT, YT
Length: 146 amino acids
Theoretical weight: 17.19 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60490 (Residues: 1-146; Coverage: 100%)
Gene names: TTHA1031, rplS
Pfam: Ribosomal protein L19
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Chains: RU, YU
Length: 118 amino acids
Theoretical weight: 13.78 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60491 (Residues: 1-118; Coverage: 100%)
Gene names: TTHA0553, rplT
Pfam: Ribosomal protein L20
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Chains: RV, YV
Length: 101 amino acids
Theoretical weight: 11.07 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60492 (Residues: 1-101; Coverage: 100%)
Gene names: TTHA1783, rplU
Pfam: Ribosomal prokaryotic L21 protein
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Chains: RX, YX
Length: 96 amino acids
Theoretical weight: 10.76 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP0 (Residues: 1-96; Coverage: 100%)
Gene names: TTHA1690, rplW
Pfam: Ribosomal protein L23
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Chains: R0, Y0
Length: 85 amino acids
Theoretical weight: 9.53 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60493 (Residues: 1-85; Coverage: 100%)
Gene names: TTHA1782, rpmA
Pfam: Ribosomal L27 protein
InterPro:

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Chains: R1, Y1
Length: 98 amino acids
Theoretical weight: 11 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60494 (Residues: 1-98; Coverage: 100%)
Gene names: TTHA0042, rpmB
Pfam: Ribosomal L28 family
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Chains: R2, Y2
Length: 72 amino acids
Theoretical weight: 8.67 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP6 (Residues: 1-72; Coverage: 100%)
Gene names: TTHA1684, rpmC
Pfam: Ribosomal L29 protein
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Chains: R5, Y5
Length: 60 amino acids
Theoretical weight: 6.72 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80339 (Residues: 1-60; Coverage: 100%)
Gene names: TTHA0418, rpl32, rpmF
Pfam: Ribosomal L32p protein family
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Chains: R7, Y7
Length: 49 amino acids
Theoretical weight: 6.13 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80340 (Residues: 1-49; Coverage: 100%)
Gene names: TTHA0446, rpl34, rpmH
Pfam: Ribosomal protein L34
InterPro:

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Chains: R9, Y9
Length: 37 amino acids
Theoretical weight: 4.44 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHR2 (Residues: 1-37; Coverage: 100%)
Gene names: TTHA1668, rpmJ
Pfam: Ribosomal protein L36
InterPro:

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Name: 16S ribosomal RNA
Representative chains: QA, XA
Length: 1508 nucleotides
Theoretical weight: 489.64 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: tRNA fMet
Representative chains: QV, QW, XV, XW
Length: 77 nucleotides
Theoretical weight: 24.8 KDa
Rfam: tRNA

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Name: Messenger RNA
Representative chains: QX, XX
Length: 25 nucleotides
Theoretical weight: 8.16 KDa

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Name: ASL Leu
Representative chains: QY, XY
Length: 17 nucleotides
Theoretical weight: 5.41 KDa

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Name: 23S ribosomal RNA
Representative chains: RA, YA
Length: 2915 nucleotides
Theoretical weight: 947.9 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: RB, YB
Length: 122 nucleotides
Theoretical weight: 39.49 KDa
Rfam: 5S ribosomal RNA

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