› 6byl

X-ray diffraction
3.35Å resolution

Structure of 14-3-3 gamma bound to O-GlcNAcylated thr peptide

Released:
DOI: 10.2210/pdb6byl/pdb
PDB entry 6byl coloured by chain, front view.
PDB entry 6byl coloured by chain, side view.
PDB entry 6byl coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins.
Toleman CA, Schumacher MA, Yu SH, Zeng W, Cox NJ, Smith TJ, Soderblom EJ, Wands AM, Kohler JJ, Boyce M
Proc Natl Acad Sci U S A 115 5956-5961 (2018)
PMID: 29784830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero trimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-158524 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein gamma Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 240 amino acids
Theoretical weight: 27.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61981 (Residues: 2-241; Coverage: 97%)
Gene name: YWHAG
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
TSASTTVPVTTATTTTTSTW O-GlcNac peptide Chains: G, P, T
Molecule details ›
Chains: G, P, T
Length: 20 amino acids
Theoretical weight: 2.02 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P41212
Unit cell:
a: 121.2Å b: 121.2Å c: 310.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.285
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Role of O-Linked N-Acetylglucosamine Protein Modification in Cellular (Patho)Physiology.
Chatham et al. (2021)
12 more

1 mention without citation

Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins.
Toleman et al. (2018)