› 6bw0

X-ray diffraction
2.1Å resolution

Nipah virus W protein C-terminus in complex with Importin alpha 1

Released:
DOI: 10.2210/pdb6bw0/pdb
PDB entry 6bw0 coloured by chain, front view.
PDB entry 6bw0 coloured by chain, side view.
PDB entry 6bw0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for importin alpha 3 specificity of W proteins in Hendra and Nipah viruses.
Smith KM, Tsimbalyuk S, Edwards MR, Cross EM, Batra J, Soares da Costa TP, Aragão D, Basler CF, Forwood JK
OpenAccess logo Nat Commun 9 3703 (2018)
PMID: 30209309

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142950 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein W Chain: C
Molecule details ›
Chain: C
Length: 41 amino acids
Theoretical weight: 4.77 KDa
Source organism: Henipavirus nipahense
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C1C7 (Residues: 411-450; Coverage: 9%)
Gene name: P/V/C
Importin subunit alpha-1 Chain: E
Molecule details ›
Chain: E
Length: 510 amino acids
Theoretical weight: 55.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 77.855Å b: 88.818Å c: 97.646Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
6 more

4 mentions without citation

The Intrinsically Disordered W Protein Is Multifunctional during Henipavirus Infection, Disrupting Host Signalling Pathways and Nuclear Import.
Tsimbalyuk et al. (2020)
3 more