X-ray diffraction
1.15Å resolution

CTX-M-27 Beta-Lactamase in Complex with a Non-Covalent Tetrazole Inhibitor

Source organism: Escherichia coli
Entry authors: Pemberton OA, Chen Y

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 27.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: B5LY47 (Residues: 17-278; Coverage: 94%)
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 44.96Å b: 107.24Å c: 47.79Å
α: 90° β: 101.86° γ: 90°
R R work R free
0.142 0.141 0.166
Expression system: Escherichia coli