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Crystal structure of AMP-PNP-bound bacterial Get3-like A and B in Mycobacterium tuberculosis

X-ray diffraction
3.1Å resolution
PDB entry 6bs5 coloured by chain, front view.
PDB entry 6bs5 coloured by chain, side view.
PDB entry 6bs5 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Anion-transporting ATPase-like domain-containing protein and Putative ATPase Rv3679
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Multimeric state: hetero dimer
Accessible surface area: 30221.61 Å2
Buried surface area: 5475.86 Å2
Dissociation area: 2,275.7 Å2
Dissociation energy (ΔGdiss): 28.4 kcal/mol
Dissociation entropy (TΔSdiss): 14.08 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-125198

Macromolecules

Putative ATPase Rv3679
Chain: A
Length: 340 amino acids
Theoretical weight: 35.89 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WKX5 (Residues: 1-340; Coverage: 100%)
Gene names: MTV025.027, Rv3679
Pfam: Anion-transporting ATPase
InterPro:
Putative ATPase Rv3679 in PDB entry 6bs5, assembly 1, front view.
Putative ATPase Rv3679 in PDB entry 6bs5, assembly 1, side view.
Putative ATPase Rv3679 in PDB entry 6bs5, assembly 1, top view.
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Anion-transporting ATPase-like domain-containing protein
Chain: B
Length: 394 amino acids
Theoretical weight: 42.52 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: I6Y498 (Residues: 1-386; Coverage: 100%)
Gene name: Rv3680
Pfam: Anion-transporting ATPase
InterPro:
Anion-transporting ATPase-like domain-containing protein in PDB entry 6bs5, assembly 1, front view.
Anion-transporting ATPase-like domain-containing protein in PDB entry 6bs5, assembly 1, side view.
Anion-transporting ATPase-like domain-containing protein in PDB entry 6bs5, assembly 1, top view.
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