6bq3

X-ray diffraction
1.91Å resolution

Crystal structure of Medicago truncatula Thermospermine Synthase (MtTSPS) in complex with 5'-S-(3-aminopropyl)-5'-thioadenosine

Released:
Source organism: Medicago truncatula
Entry authors: Sekula B, Dauter Z

Function and Biology Details

Reaction catalysed:
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PABS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 37.28 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G7K2D1 (Residues: 1-328; Coverage: 100%)
Gene names: 11439099, MTR_5g006140, MtrunA17_Chr5g0394371
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 81.052Å b: 81.052Å c: 163.128Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.238
Expression system: Escherichia coli BL21(DE3)