› 6bp2

X-ray diffraction
3.17Å resolution

Therapeutic human monoclonal antibody MR191 bound to a marburgvirus glycoprotein

Released:
DOI: 10.2210/pdb6bp2/pdb
PDB entry 6bp2 coloured by chain, front view.
PDB entry 6bp2 coloured by chain, side view.
PDB entry 6bp2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Marburgvirus-Neutralizing Human Monoclonal Antibody MR191 Targets a Conserved Site to Block Virus Receptor Binding.
King LB, Fusco ML, Flyak AI, Ilinykh PA, Huang K, Gunn B, Kirchdoerfer RN, Hastie KM, Sangha AK, Meiler J, Alter G, Bukreyev A, Crowe JE, Saphire EO
Cell Host Microbe 23 101-109.e4 (2018)
PMID: 29324225

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231367 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
GP1 Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 27.89 KDa
UniProt:
  • Canonical: Q1PDC7 (Residues: 17-259; Coverage: 36%)
Gene name: GP
Sequence domains: Filovirus glycoprotein
GP2 Chain: B
Molecule details ›
Chain: B
Length: 202 amino acids
Theoretical weight: 22.56 KDa
UniProt:
  • Canonical: Q1PDC7 (Residues: 436-637; Coverage: 31%)
Gene name: GP
MR191 Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 229 amino acids
Theoretical weight: 24.38 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
Structure domains: Immunoglobulins
MR191 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 22.85 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P321
Unit cell:
a: 133.796Å b: 133.796Å c: 151.003Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.252 0.284
Expression system: Nicotiana benthamiana

Quick links

Citations

7 review citations

Antibody responses to viral infections: a structural perspective across three different enveloped viruses.
Murin et al. (2019)
6 more

10 mentions without citation

The structural basis for filovirus neutralization by monoclonal antibodies.
King et al. (2018)
9 more