6bp1

X-ray diffraction
2Å resolution

Crystal structure of human KRAS A59G mutant in complex with GCP

Released:
Source organism: Homo sapiens
Entry authors: Bera AK, Lu J, Westover KD

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed; Isoform 2B of GTPase KRas Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-169; Coverage: 89%)
  • Best match: P01116-2 (Residues: 1-169)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 34.391Å b: 47.473Å c: 89.665Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.223
Expression system: Escherichia coli