6bof

X-ray diffraction
1.4Å resolution

Crystal structure of KRAS A146T-GDP demonstrating open switch 1 conformation

Released:

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed; Isoform 2B of GTPase KRas Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 19.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01116 (Residues: 2-169; Coverage: 89%)
  • Best match: P01116-2 (Residues: 2-169)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3
Unit cell:
a: 78.024Å b: 78.024Å c: 55.863Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.113 0.112 0.145
Expression system: Escherichia coli BL21(DE3)