› 6bn0

X-ray diffraction
1.95Å resolution

Avirulence protein 4 (Avr4) from Cladosporium fulvum bound to the hexasaccharide of chitin

Released:
DOI: 10.2210/pdb6bn0/pdb
PDB 6bn0 coloured by chain and viewed from the front.
PDB 6bn0 coloured by chain and viewed from the side.
PDB 6bn0 coloured by chain and viewed from the top.
Source organism: Passalora fulva
Primary publication:
Structure of the Cladosporium fulvum Avr4 effector in complex with (GlcNAc)6 reveals the ligand-binding mechanism and uncouples its intrinsic function from recognition by the Cf-4 resistance protein.
Hurlburt NK, Chen LH, Stergiopoulos I, Fisher AJ
PLoS Pathog. 14 e1007263 (2018)
PMID: 30148881

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Race-specific elicitor A4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 79 amino acids
Theoretical weight: 8.83 KDa
Source organism: Passalora fulva
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00363 (Residues: 35-113; Coverage: 68%)
Gene name: AVR4
Sequence domains: Chitin binding Peritrophin-A domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 39.864Å b: 41.081Å c: 121.365Å
α: 90° β: 97.87° γ: 90°
R-values:
R R work R free
0.17 0.168 0.214
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Fungal effectors, the double edge sword of phytopathogens.
Pradhan et al. (2021)
2 more

1 mention without citation

NMR and Fluorescence Spectroscopies Reveal the Preorganized Binding Site in Family 14 Carbohydrate-Binding Module from Human Chitotriosidase.
Madland et al. (2019)