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Structural Insights into the Lipid A Transport Pathway in MsbA.

Padayatti PS, Lee SC, Stanfield RL, Wen PC, Tajkhorshid E, Wilson IA, Zhang Q
Structure 27 1114-1123.e3 (2019)
Cited: 23 times
EuropePMC logo PMID: 31130486

Abstract

MsbA is an essential ATP-binding cassette transporter in Gram-negative bacteria that transports lipid A and lipopolysaccharide from the cytoplasmic leaflet to the periplasmic leaflet of the inner membrane. Here we report the X-ray structure of MsbA from Salmonella typhimurium at 2.8-Å resolution in an inward-facing conformation after cocrystallization with lipid A and using a stabilizing facial amphiphile. The structure displays a large amplitude opening in the transmembrane portal, which is likely required for lipid A to pass from its site of synthesis into the protein-enclosed transport pathway. Putative lipid A density is observed further inside the transmembrane cavity, consistent with a trap and flip model. Additional electron density attributed to lipid A is observed near an outer surface cleft at the periplasmic ends of the transmembrane helices. These findings provide new structural insights into the lipid A transport pathway through comparative analysis with existing MsbA structures.

Reviews - 6bl6 mentioned but not cited (2)

  1. Structural and functional diversity calls for a new classification of ABC transporters. Thomas C, Aller SG, Beis K, Carpenter EP, Chang G, Chen L, Dassa E, Dean M, Duong Van Hoa F, Ekiert D, Ford R, Gaudet R, Gong X, Holland IB, Huang Y, Kahne DK, Kato H, Koronakis V, Koth CM, Lee Y, Lewinson O, Lill R, Martinoia E, Murakami S, Pinkett HW, Poolman B, Rosenbaum D, Sarkadi B, Schmitt L, Schneider E, Shi Y, Shyng SL, Slotboom DJ, Tajkhorshid E, Tieleman DP, Ueda K, Váradi A, Wen PC, Yan N, Zhang P, Zheng H, Zimmer J, Tampé R. FEBS Lett 594 3767-3775 (2020)
  2. The Switch and Reciprocating Models for the Function of ABC Multidrug Exporters: Perspectives on Recent Research. Jones PM, George AM. Int J Mol Sci 24 2624 (2023)

Articles - 6bl6 mentioned but not cited (5)

  1. Structural Insights into the Lipid A Transport Pathway in MsbA. Padayatti PS, Lee SC, Stanfield RL, Wen PC, Tajkhorshid E, Wilson IA, Zhang Q. Structure 27 1114-1123.e3 (2019)
  2. ABCA1 is an extracellular phospholipid translocase. Segrest JP, Tang C, Song HD, Jones MK, Davidson WS, Aller SG, Heinecke JW. Nat Commun 13 4812 (2022)
  3. Structural basis for lipid and copper regulation of the ABC transporter MsbA. Lyu J, Liu C, Zhang T, Schrecke S, Elam NP, Packianathan C, Hochberg GKA, Russell D, Zhao M, Laganowsky A. Nat Commun 13 7291 (2022)
  4. The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells. Galazzo L, Meier G, Januliene D, Parey K, De Vecchis D, Striednig B, Hilbi H, Schäfer LV, Kuprov I, Moeller A, Bordignon E, Seeger MA. Sci Adv 8 eabn6845 (2022)
  5. The Alternating Access Mechanism in Mammalian Multidrug Resistance Transporters and Their Bacterial Homologs. Badiee SA, Isu UH, Khodadadi E, Moradi M. Membranes (Basel) 13 568 (2023)


Reviews citing this publication (3)

  1. Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases. Kim J, Cater RJ, Choy BC, Mancia F. J Mol Biol 433 167005 (2021)
  2. Phospholipid Scrambling by G Protein-Coupled Receptors. Khelashvili G, Menon AK. Annu Rev Biophys 51 39-61 (2022)
  3. Checkpoints That Regulate Balanced Biosynthesis of Lipopolysaccharide and Its Essentiality in Escherichia coli. Klein G, Wieczorek A, Szuster M, Raina S. Int J Mol Sci 23 189 (2021)

Articles citing this publication (13)

  1. Molecular Basis of Essentiality of Early Critical Steps in the Lipopolysaccharide Biogenesis in Escherichia coli K-12: Requirement of MsbA, Cardiolipin, LpxL, LpxM and GcvB. Gorzelak P, Klein G, Raina S. Int J Mol Sci 22 5099 (2021)
  2. Distinct allosteric mechanisms of first-generation MsbA inhibitors. Thélot FA, Zhang W, Song K, Xu C, Huang J, Liao M. Science 374 580-585 (2021)
  3. Energetics of lipid transport by the ABC transporter MsbA is lipid dependent. Guo D, Singh H, Shimoyama A, Guffick C, Tang Y, Rowe SM, Noel T, Spring DR, Fukase K, van Veen HW. Commun Biol 4 1379 (2021)
  4. Effects of Verapamil and Two Bisbenzylisoquinolines, Curine and Guattegaumerine Extracted from Isolona hexaloba, on the Inhibition of ABC Transporters from Pseudomonas aeruginosa. Hulen C, Racine PJ, Feuilloley M, Elomri A, Lomri NE. Antibiotics (Basel) 11 700 (2022)
  5. A New Factor LapD Is Required for the Regulation of LpxC Amounts and Lipopolysaccharide Trafficking. Wieczorek A, Sendobra A, Maniyeri A, Sugalska M, Klein G, Raina S. Int J Mol Sci 23 9706 (2022)
  6. Catalytically Cleavable Detergent for Membrane Protein Studies. Liu L, Zhu Z, Zhou F, Xue D, Hu T, Luo W, Qiu Y, Wu D, Zhao F, Le Z, Tao H. ACS Omega 6 21087-21093 (2021)
  7. Production of selenium nanoparticles occurs through an interconnected pathway of sulphur metabolism and oxidative stress response in Pseudomonas putida KT2440. Avendaño R, Muñoz-Montero S, Rojas-Gätjens D, Fuentes-Schweizer P, Vieto S, Montenegro R, Salvador M, Frew R, Kim J, Chavarría M, Jiménez JI. Microb Biotechnol 16 931-946 (2023)
  8. Cryo-EM Analysis of the Lipopolysaccharide Flippase MsbA. Thélot FA, Liao M. Methods Mol Biol 2548 233-247 (2022)
  9. Drug sensitivity and genome-wide analysis of two strains of Mycoplasma gallisepticum with different biofilm intensity. Ma X, Wang L, Yang F, Li J, Guo L, Guo Y, He S. Front Microbiol 14 1196747 (2023)
  10. Facade-Based Bicelles as a New Tool for Production of Active Membrane Proteins in a Cell-Free System. Goncharuk MV, Vasileva EV, Ananiev EA, Gorokhovatsky AY, Bocharov EV, Mineev KS, Goncharuk SA. Int J Mol Sci 24 14864 (2023)
  11. Identification of a muropeptide precursor transporter from gut microbiota and its role in preventing intestinal inflammation. Liuu S, Nepelska M, Pfister H, Gamelas Magalhaes J, Chevalier G, Strozzi F, Billerey C, Maresca M, Nicoletti C, Di Pasquale E, Pechard C, Bardouillet L, Girardin SE, Boneca IG, Doré J, Blottière HM, Bonny C, Chene L, Cultrone A. Proc Natl Acad Sci U S A 120 e2306863120 (2023)
  12. Editorial Lipopolysaccharides: Regulated Biosynthesis and Structural Diversity. Raina S. Int J Mol Sci 24 7498 (2023)
  13. Probing the allosteric NBD-TMD crosstalk in the ABC transporter MsbA by solid-state NMR. Novischi SYP, Karoly-Lakatos A, Chok K, Bonifer C, Becker-Baldus J, Glaubitz C. Commun Biol 7 43 (2024)


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