6bl6

X-ray diffraction
2.8Å resolution

Crystallization of lipid A transporter MsbA from Salmonella typhimurium

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + lipid A-core oligosaccharide(Side 1) = ADP + phosphate + lipid A-core oligosaccharide(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipid A export ATP-binding/permease protein MsbA Chains: A, B
Molecule details ›
Chains: A, B
Length: 576 amino acids
Theoretical weight: 63.61 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63359 (Residues: 7-582; Coverage: 99%)
Gene names: STM0984, msbA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 82.052Å b: 154.819Å c: 228.886Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.27 0.301
Expression system: Escherichia coli BL21(DE3)