› 6bk0

X-ray diffraction
1.47Å resolution

Crystal structure of Os79 Q202A from O. sativa in complex with UDP.

Released:
DOI: 10.2210/pdb6bk0/pdb
PDB entry 6bk0 coloured by chain, front view.
PDB entry 6bk0 coloured by chain, side view.
PDB entry 6bk0 coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Determinants and Expansion of Specificity in a Trichothecene UDP-Glucosyltransferase from Oryza sativa.
Wetterhorn KM, Gabardi K, Michlmayr H, Malachova A, Busman M, McCormick SP, Berthiller F, Adam G, Rayment I
Biochemistry 56 6585-6596 (2017)
PMID: 29140092

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glycosyltransferase 79 Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 51.09 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q7XT97 (Residues: 1-466; Coverage: 100%)
Gene names: LOC_Os04g12970, OS79, OSJNBa0052O21.15, Os04g0206600, OsJ_13826, UGT79
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 59.409Å b: 83.233Å c: 99.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.181
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

4 review citations

Enzymes for Detoxification of Various Mycotoxins: Origins and Mechanisms of Catalytic Action.
Lyagin et al. (2019)
3 more