› 6bja

X-ray diffraction
1.6Å resolution

Crystal structure of Acat5 thiolase from Ascaris suum in complex with coenzyme A

Released:
DOI: 10.2210/pdb6bja/pdb
PDB 6bja coloured by chain and viewed from the front.
PDB 6bja coloured by chain and viewed from the side.
PDB 6bja coloured by chain and viewed from the top.
Source organism: Ascaris suum
Entry authors: Blaisse MR, Fu B, Chang MCY

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase A Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 41.99 KDa
Source organism: Ascaris suum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F1L3N8 (Residues: 28-421; Coverage: 94%)
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand COA 1 x COA
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I222
Unit cell:
a: 71.662Å b: 111.78Å c: 112.756Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.173
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

The 3-ketoacyl-CoA thiolase: an engineered enzyme for carbon chain elongation of chemical compounds.
Liu et al. (2020)
2 other articles