› 6bfy

X-ray diffraction
1.81Å resolution

Crystal structure of enolase from Escherichia coli with bound 2-phosphoglycerate substrate

Released:
DOI: 10.2210/pdb6bfy/pdb
PDB entry 6bfy coloured by chain, front view.
PDB entry 6bfy coloured by chain, side view.
PDB entry 6bfy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural and Functional Studies of Bacterial Enolase, a Potential Target against Gram-Negative Pathogens.
Krucinska J, Falcone E, Erlandsen H, Hazeen A, Lombardo MN, Estrada A, Robinson VL, Anderson AC, Wright DL
Biochemistry 58 1188-1197 (2019)
PMID: 30714720

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 449 amino acids
Theoretical weight: 47.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6P9 (Residues: 1-432; Coverage: 100%)
Gene names: JW2750, b2779, eno
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand 2PG 6 x 2PG
Ligand MG 12 x MG
Ligand SO4 22 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P212121
Unit cell:
a: 105.94Å b: 142.4Å c: 206.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.166 0.232
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Enolase 1, a Moonlighting Protein, as a Potential Target for Cancer Treatment.
Qiao et al. (2021)
1 more

3 mentions without citation

Structural and Functional Studies of Bacterial Enolase, a Potential Target against Gram-Negative Pathogens.
Krucinska et al. (2019)
2 more