6bf4

X-ray diffraction
2.38Å resolution

Crystal Structure of HIV-1 Clade AE Strain CNE55 gp120 Core in Complex with Neutralizing Antibody VRC-PG05 that Targets the Center of the Silent Face on the Outer Domain of gp120

Released:
Entry authors: Zhou T, Kwong PD

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (10 distinct):
Envelope glycoprotein gp160 Chains: A, G
Molecule details ›
Chains: A, G
Length: 344 amino acids
Theoretical weight: 38.22 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: D7S2G1 (Residues: 43-474; Coverage: 41%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
VRC-PG05 Fab heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 229 amino acids
Theoretical weight: 24.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
VRC-PG05 Fab light chain Chains: C, L
Molecule details ›
Chains: C, L
Length: 219 amino acids
Theoretical weight: 24.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer : NEW Components: NAG, MAN
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 231.438Å b: 89.255Å c: 123.407Å
α: 90° β: 119.15° γ: 90°
R-values:
R R work R free
0.194 0.192 0.228
Expression system: Homo sapiens