PDBe 6bcb

X-ray diffraction
1.4Å resolution

A Complex between PH Domain of p114RhoGEF and Activated RhoA Bound to a GTP Analog

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transforming protein RhoA Chain: F
Molecule details ›
Chain: F
Length: 185 amino acids
Theoretical weight: 20.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Rho guanine nucleotide exchange factor 18 Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P9R4 (Residues: 686-828; Coverage: 10%)
Gene names: Arhgef18, Kiaa0521

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 48.934Å b: 60.039Å c: 65.475Å
α: 90° β: 108.14° γ: 90°
R-values:
R R work R free
0.138 0.136 0.165
Expression system: Escherichia coli BL21(DE3)