PDBe 6bc0

X-ray diffraction
2.2Å resolution

A Complex between PH Domain of p190RhoGEF and Activated RhoA Bound to a GTP Analog

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 28 Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 17.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N1W1 (Residues: 1049-1194; Coverage: 9%)
Gene names: ARHGEF28, KIAA1998, RGNEF
Sequence domains: PH domain
Transforming protein RhoA Chain: F
Molecule details ›
Chain: F
Length: 185 amino acids
Theoretical weight: 20.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 73.16Å b: 73.16Å c: 266.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.281
Expression system: Escherichia coli BL21(DE3)