6bbm

Electron Microscopy
4.1Å resolution

Mechanisms of Opening and Closing of the Bacterial Replicative Helicase: The DnaB Helicase and Lambda P Helicase Loader Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Replicative DNA helicase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 471 amino acids
Theoretical weight: 52.45 KDa
Source organism: Escherichia coli O111:NM
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACB0 (Residues: 1-471; Coverage: 100%)
Gene names: JW4012, b4052, dnaB, groP, grpA
Sequence domains:
Replication protein P Chains: V, W, X, Y, Z
Molecule details ›
Chains: V, W, X, Y, Z
Length: 233 amino acids
Theoretical weight: 23.14 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03689 (Residues: 1-107; Coverage: 46%)
Gene name: P

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-7076
Expression system: Escherichia coli BL21(DE3)