› 6bag

X-ray diffraction
2.4Å resolution

Lactate Dehydrogenase in complex with inhibitor (R)-5-((2-chlorophenyl)thio)-6'-((4-fluorophenyl)amino)-4-hydroxy-2-(thiophen-3-yl)-2,3-dihydro-[2,2'-bipyridin]-6(1H)-one

Released:
DOI: 10.2210/pdb6bag/pdb
PDB entry 6bag coloured by chain, front view.
PDB entry 6bag coloured by chain, side view.
PDB entry 6bag coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ultsch M, Eigenbrot C

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 331 amino acids
Theoretical weight: 36.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00338 (Residues: 2-332; Coverage: 100%)
Gene names: LDHA, PIG19
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
3 bound ligands:
Ligand EPE 3 x EPE
Ligand SO4 4 x SO4
Ligand D0V 4 x D0V
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 78.799Å b: 81.007Å c: 102.756Å
α: 90° β: 98.53° γ: 90°
R-values:
R R work R free
0.176 0.175 0.213
Expression system: Escherichia coli

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