› 6bad

X-ray diffraction
2.1Å resolution

Lactate Dehydrogenase in complex with inhibitor (R)-3-((2-chlorophenyl)thio)-6-(3-((4-fluorophenyl)amino)phenyl)-4-hydroxy-6-(thiophen-3-yl)-5,6-dihydro-2H-pyran-2-one

Released:
DOI: 10.2210/pdb6bad/pdb
PDB 6bad coloured by chain and viewed from the front.
PDB 6bad coloured by chain and viewed from the side.
PDB 6bad coloured by chain and viewed from the top.
Source organism: Homo sapiens
Entry authors: Ultsch M, Eigenbrot C

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 331 amino acids
Theoretical weight: 36.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00338 (Residues: 2-332; Coverage: 100%)
Gene names: LDHA, PIG19
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
3 bound ligands:
Ligand EPE 7 x EPE
Ligand SO4 5 x SO4
Ligand D0Y 4 x D0Y
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 75.401Å b: 80.714Å c: 102.429Å
α: 90° β: 96.81° γ: 90°
R-values:
R R work R free
0.194 0.194 0.23
Expression system: Escherichia coli

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