X-ray diffraction
2.1Å resolution

Crystal Structure of the N-terminal domain of human METTL16 in complex with SAH

Source organism: Homo sapiens
Entry authors: Ruszkowska A, Ruszkowski M, Dauter Z, Brown JA

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in mRNA
S-adenosyl-L-methionine + adenine in U6 snRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in U6 snRNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
RNA N6-adenosine-methyltransferase METTL16 Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Canonical: Q86W50 (Residues: 1-291; Coverage: 52%)
Gene names: METT10D, METTL16
Sequence domains: RNA methyltransferase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I4132
Unit cell:
a: 190.351Å b: 190.351Å c: 190.351Å
α: 90° β: 90° γ: 90°
R R work R free
0.182 0.181 0.219
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'