› 6b92

X-ray diffraction
2.1Å resolution

Crystal Structure of the N-terminal domain of human METTL16 in complex with SAH

Released:
DOI: 10.2210/pdb6b92/pdb
PDB 6b92 coloured by chain and viewed from the front.
PDB 6b92 coloured by chain and viewed from the side.
PDB 6b92 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Entry authors: Ruszkowska A, Ruszkowski M, Dauter Z, Brown JA

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in mRNA
S-adenosyl-L-methionine + adenine in U6 snRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in U6 snRNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA N6-adenosine-methyltransferase METTL16 Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q86W50 (Residues: 1-291; Coverage: 52%)
Gene names: METT10D, METTL16
Sequence domains: RNA methyltransferase

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I4132
Unit cell:
a: 190.351Å b: 190.351Å c: 190.351Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.219
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

19 review citations

A comprehensive review of m6A/m6Am RNA methyltransferase structures.
Oerum et al. (2021)
18 more