› 6b5y

X-ray diffraction
2.75Å resolution

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161732 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 28.4 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WKD3 (Residues: 41-307; Coverage: 98%)
Gene names: MTCY49.07c, Rv2068c, blaA, blaC
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand 9F2 4 x 9F2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P21
Unit cell:
a: 78.53Å b: 96.03Å c: 112.58Å
α: 90° β: 109.68° γ: 90°
R-values:
R R work R free
0.196 0.193 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
17 more

2 mentions without citation

Protein Data Bank: A Comprehensive Review of 3D Structure Holdings and Worldwide Utilization by Researchers, Educators, and Students.
Burley et al. (2022)
1 more