› 6b4j

X-ray diffraction
3.4Å resolution

Crystal structure of human Gle1 CTD-Nup42 GBM-DDX19B(AMPPNP) complex

Released:
DOI: 10.2210/pdb6b4j/pdb
PDB 6b4j coloured by chain and viewed from the front.
PDB 6b4j coloured by chain and viewed from the side.
PDB 6b4j coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of mRNA export regulation by the nuclear pore complex.
Lin DH, Correia AR, Cai SW, Huber FM, Jette CA, Hoelz A
OpenAccess logo Nat Commun 9 2319 (2018)
PMID: 29899397

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nucleoporin GLE1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 36.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53GS7 (Residues: 382-698; Coverage: 45%)
Gene names: GLE1, GLE1L
Sequence domains: GLE1-like protein
Nucleoporin NUP42 Chains: C, D
Molecule details ›
Chains: C, D
Length: 49 amino acids
Theoretical weight: 5.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15504 (Residues: 376-422; Coverage: 11%)
Gene names: CG1, NUP42, NUPL2
ATP-dependent RNA helicase DDX19B Chains: E, F
Molecule details ›
Chains: E, F
Length: 430 amino acids
Theoretical weight: 48.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UMR2 (Residues: 54-479; Coverage: 89%)
Gene names: DBP5, DDX19, DDX19B, TDBP
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand PO4 6 x PO4
Ligand MG 2 x MG
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P21
Unit cell:
a: 87.94Å b: 73.56Å c: 145.86Å
α: 90° β: 95.07° γ: 90°
R-values:
R R work R free
0.266 0.261 0.313
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Gonadotropin Regulation Testicular RNA Helicase, Two Decades of Studies on Its Structure Function and Regulation From Its Discovery Opens a Window for Development of a Non-hormonal Oral Male Contraceptive.
Dufau et al. (2019)
1 more

1 mention without citation

The Structure of the Nuclear Pore Complex (An Update).
Lin et al. (2019)