6b4i

X-ray diffraction
3.62Å resolution

Crystal structure of human Gle1 CTD-Nup42 GBM-DDX19B(ADP) complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nucleoporin GLE1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 36.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53GS7 (Residues: 382-698; Coverage: 45%)
Gene names: GLE1, GLE1L
Sequence domains: GLE1-like protein
Nucleoporin NUP42 Chains: C, D
Molecule details ›
Chains: C, D
Length: 50 amino acids
Theoretical weight: 5.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15504 (Residues: 381-423; Coverage: 10%)
Gene names: CG1, NUP42, NUPL2
ATP-dependent RNA helicase DDX19B Chains: E, F
Molecule details ›
Chains: E, F
Length: 430 amino acids
Theoretical weight: 48.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UMR2 (Residues: 54-479; Coverage: 89%)
Gene names: DBP5, DDX19, DDX19B, TDBP
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 87.74Å b: 74.65Å c: 146.83Å
α: 90° β: 94.85° γ: 90°
R-values:
R R work R free
0.239 0.234 0.281
Expression system: Escherichia coli