› 6b2o

X-ray diffraction
2.35Å resolution

LarE, a sulfur transferase involved in synthesis of the cofactor for lactate racemase, C176A variant

Released:
DOI: 10.2210/pdb6b2o/pdb
PDB entry 6b2o coloured by chain, front view.
PDB entry 6b2o coloured by chain, side view.
PDB entry 6b2o coloured by chain, top view.
Source organism: Lactiplantibacillus plantarum
Primary publication:
Analysis of the Active Site Cysteine Residue of the Sacrificial Sulfur Insertase LarE from Lactobacillus plantarum.
Fellner M, Rankin JA, Desguin B, Hu J, Hausinger RP
Biochemistry 57 5513-5523 (2018)
PMID: 30157639

Function and Biology Details

Reaction catalysed: 
(1a) ATP + pyridinium-3,5-dicarboxylate mononucleotide = diphosphate + 5-carboxy-1-(5-O-phospho-beta-D-ribofuranosyl)pyridinium-3-carbonyl adenylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123801 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 286 amino acids
Theoretical weight: 31.69 KDa
Source organism: Lactiplantibacillus plantarum
Expression system: Escherichia coli
UniProt:
  • Canonical: F9UST4 (Residues: 1-276; Coverage: 100%)
Gene names: larE, lp_0109
Sequence domains: NAD synthase

Ligands and Environments

2 bound ligands:
Ligand PO4 6 x PO4
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P4122
Unit cell:
a: 108.66Å b: 108.66Å c: 328.666Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.244
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure, function, and biosynthesis of nickel-dependent enzymes.
Alfano et al. (2020)
2 more