› 6b2d

X-ray diffraction
3.01Å resolution

Crystal structure of fluoride channel Fluc Ec2 T114S Mutant

Released:
DOI: 10.2210/pdb6b2d/pdb
PDB entry 6b2d coloured by chain, front view.
PDB entry 6b2d coloured by chain, side view.
PDB entry 6b2d coloured by chain, top view.
Source organisms:
Primary publication:
Molecular determinants of permeation in a fluoride-specific ion channel.
Last NB, Sun S, Pham MC, Miller C
Elife 6 (2017)
PMID: 28952925

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166446 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fluoride-specific ion channel FluC Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6J5N4 (Residues: 1-126; Coverage: 100%)
Gene names: AKG29_01220, B6R31_005152, BANRA_05655, BK292_28205, C2M16_27610, CF22_004922, D4U49_23430, D9J61_22795, DNQ45_14105, ECS286_0026, EIA08_25210, EIZ93_19245, EN85_004657, EPS76_20210, EcSP15_n5000, ExPECSC038_03841, F9413_22620, FZU14_25965, G3W53_24200, GAI89_25135, HIE29_005335, HL601_24225, HLZ50_24015, NCTC9001_00147, NCTC9077_06349, RCS79_P0115, SAMEA4370473_00090, crcB, crcB_2, flc_2, fluC, pCTXM15_EC8_00123, pO103_22
Sequence domains: CrcB-like protein, Camphor Resistance (CrcB)
monobody Chains: C, D
Molecule details ›
Chains: C, D
Length: 96 amino acids
Theoretical weight: 10.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand DMU 3 x DMU
Ligand F 3 x F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41
Unit cell:
a: 87.09Å b: 87.09Å c: 143.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Membrane Exporters of Fluoride Ion.
McIlwain et al. (2021)
9 other articles

1 mention without citation

Molecular determinants of permeation in a fluoride-specific ion channel.
Last et al. (2017)