Function and Biology

TylHI in complex with native substrate 23-deoxy-5-O-mycaminosyl-tylonolide (23-DMTL)

Source organism: Streptomyces fradiae
Biochemical function: oxidoreductase activity
Biological process: antibiotic biosynthetic process
Cellular component: not assigned

EC 1.14.15.34: 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase

Reaction catalysed:
20-oxo-5-O-beta-mycaminosyltylactone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = 5-O-beta-mycaminosyltylonolide + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
Systematic name:
20-oxo-5-O-beta-mycaminosyltylactone,reduced ferredoxin:oxygen oxidoreductase (23-hydroxylating)
Alternative Name(s):
  • TylH1 (gene name)

Sequence family

Pfam Protein family (Pfam)
PF00067
Domain description: Cytochrome P450
Occurring in:
  1. 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase
1 copy of Pfam domain PF00067 (Cytochrome P450) in 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase in PDB 6b11.

InterPro InterPro annotations
IPR002397
Domain description: Cytochrome P450, B-class
Occurring in:
  1. 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase
IPR001128
Domain description: Cytochrome P450
Occurring in:
  1. 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase
IPR036396
Domain description: Cytochrome P450 superfamily
Occurring in:
  1. 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase

Structure domain

CATH CATH domain
1.10.630.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Cytochrome p450
Homology: Cytochrome P450
Occurring in:
  1. 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase
1 copy of CATH domain 1.10.630.10 (Cytochrome p450) in 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase in PDB 6b11.