6b11

X-ray diffraction
1.99Å resolution

TylHI in complex with native substrate 23-deoxy-5-O-mycaminosyl-tylonolide (23-DMTL)

Released:
Source organism: Streptomyces fradiae
Entry authors: DeMars MD, Sherman DH, Podust LM

Function and Biology Details

Reaction catalysed:
20-oxo-5-O-beta-mycaminosyltylactone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = 5-O-beta-mycaminosyltylonolide + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
20-oxo-5-O-mycaminosyltylactone 23-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 47.8 KDa
Source organism: Streptomyces fradiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZHQ1 (Residues: 1-420; Coverage: 100%)
Gene names: tylH1, tylHI
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 63.73Å b: 109.24Å c: 150.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.24
Expression system: Escherichia coli