› 6azy

X-ray diffraction
2.7Å resolution

Crystal structure of Hsp104 R328M/R757M mutant from Calcarisporiella thermophila

Released:
DOI: 10.2210/pdb6azy/pdb
PDB 6azy coloured by chain and viewed from the front.
PDB 6azy coloured by chain and viewed from the side.
PDB 6azy coloured by chain and viewed from the top.
Source organism: Calcarisporiella thermophila
Entry authors: Michalska K, Bigelow L, Hatzos-Skintges C, Jedrzejczak R, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Clp R domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 885 amino acids
Theoretical weight: 99.06 KDa
Source organism: Calcarisporiella thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A452CSQ7 (Residues: 1-885; Coverage: 100%)
Gene name: Calth2p4_003362
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 139.173Å b: 139.173Å c: 97.06Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.249
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Amyloid Fragmentation and Disaggregation in Yeast and Animals.
Kushnirov et al. (2021)
2 more