› 6avn

X-ray diffraction
2.5Å resolution

Crystal structure of unbound anti-HIV antibody Fab PGV19 at 2.5 A

Released:
DOI: 10.2210/pdb6avn/pdb
PDB 6avn coloured by chain and viewed from the front.
PDB 6avn coloured by chain and viewed from the side.
PDB 6avn coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.
Sarkar A, Bale S, Behrens AJ, Kumar S, Sharma SK, de Val N, Pallesen J, Irimia A, Diwanji DC, Stanfield RL, Ward AB, Crispin M, Wyatt RT, Wilson IA
OpenAccess logo Nat Commun 9 1956 (2018)
PMID: 29769533

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PGV19 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 24.25 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
PGV19 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 209 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 69.783Å b: 69.783Å c: 193.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.25
Expression system: Homo sapiens

Quick links

Citations

9 review citations

A Structural Update of Neutralizing Epitopes on the HIV Envelope, a Moving Target.
Parker Miller et al. (2021)
8 more