Structure analysis

CryoEM structure of E.coli his pause elongation complex

Electron Microscopy
3.8Å resolution
Source organism: Escherichia coli K-12
Assembly composition:
hetero octamer (preferred)
Entry contents: 4 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero octamer
Accessible surface area: 149555.82 Å2
Buried surface area: 39741.03 Å2
Dissociation area: 3,398.72 Å2
Dissociation energy (ΔGdiss): 30.67 kcal/mol
Dissociation entropy (TΔSdiss): 14.7 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-119586

Macromolecules

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Chain: K
Length: 84 amino acids
Theoretical weight: 9.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 1-84; Coverage: 92%)
Gene names: JW3624, b3649, rpoZ
Pfam: RNA polymerase Rpb6
InterPro:
CATH: Eukaryotic RPB6 RNA polymerase subunit

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Name: DNA (32-MER)
Representative chains: A
Length: 32 nucleotides
Theoretical weight: 9.88 KDa

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Name: DNA (32-MER)
Representative chains: B
Length: 32 nucleotides
Theoretical weight: 9.71 KDa

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Name: RNA (29-MER)
Representative chains: R
Length: 29 nucleotides
Theoretical weight: 9.23 KDa

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