› 6apb

X-ray diffraction
3Å resolution

Crystal Structure of Non-Neutralizing Infant Antibody ADI-14359 in Complex with Postfusion RSV F Glycoprotein

Released:
DOI: 10.2210/pdb6apb/pdb
PDB entry 6apb coloured by chain, front view.
PDB entry 6apb coloured by chain, side view.
PDB entry 6apb coloured by chain, top view.
Source organisms:
Primary publication:
Infants Infected with Respiratory Syncytial Virus Generate Potent Neutralizing Antibodies that Lack Somatic Hypermutation.
Goodwin E, Gilman MSA, Wrapp D, Chen M, Ngwuta JO, Moin SM, Bai P, Sivasubramanian A, Connor RI, Wright PF, Graham BS, McLellan JS, Walker LM
Immunity 48 339-349.e5 (2018)
PMID: 29396163

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230771 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fusion glycoprotein F0 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 510 amino acids
Theoretical weight: 56.76 KDa
Source organism: Human orthopneumovirus
Expression system: Homo sapiens
UniProt:
  • Canonical: P03420 (Residues: 1-513; Coverage: 87%)
Gene name: F
Sequence domains: Fusion glycoprotein F0
IgG H chain Chain: H
Molecule details ›
Chain: H
Length: 230 amino acids
Theoretical weight: 24.82 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
ADI-14359 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.28 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P212121
Unit cell:
a: 88.45Å b: 99.04Å c: 323.309Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.255
Expression system: Homo sapiens

Quick links

Citations

24 review citations

Development of therapeutic antibodies for the treatment of diseases.
Lu et al. (2020)
23 more

5 mentions without citation

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
4 more