› 6aob

X-ray diffraction
1.7Å resolution

Crystal structure of non-canonical dimeric guanylyl cyclase domain of RhoGC fusion protein from the aquatic fungus Blastocladiella emersonii

Released:
DOI: 10.2210/pdb6aob/pdb
PDB entry 6aob coloured by chain, front view.
PDB entry 6aob coloured by chain, side view.
PDB entry 6aob coloured by chain, top view.
Source organism: Blastocladiella emersonii
Primary publication:
Structure and monomer/dimer equilibrium for the guanylyl cyclase domain of the optogenetics protein RhoGC.
Kumar RP, Morehouse BR, Fofana J, Trieu MM, Zhou DH, Lorenz MO, Oprian DD
J Biol Chem 292 21578-21589 (2017)
PMID: 29118188

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate cyclase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.5 KDa
Source organism: Blastocladiella emersonii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A060H1D7 (Residues: 443-626; Coverage: 29%)
Gene name: gc1
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2221
Unit cell:
a: 91.769Å b: 95.88Å c: 100.049Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Redox regulation of soluble guanylyl cyclase.
Shah et al. (2018)
2 more

3 mentions without citation

Structure and monomer/dimer equilibrium for the guanylyl cyclase domain of the optogenetics protein RhoGC.
Kumar et al. (2017)
2 more