› 6anv

X-ray diffraction
2.26Å resolution

Crystal structure of anti-CRISPR protein AcrF1

Released:
DOI: 10.2210/pdb6anv/pdb
PDB 6anv coloured by chain and viewed from the front.
PDB 6anv coloured by chain and viewed from the side.
PDB 6anv coloured by chain and viewed from the top.
Source organisms:
Entry authors: Yang H, Patel DJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 49.77 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: L7P7M1 (Residues: 1-78; Coverage: 100%)
  • Canonical: P0AEY0 (Residues: 27-396; Coverage: 100%)
Gene names: ECs5017, JBD30_035, Z5632, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
4 bound ligands:
Ligand MES 2 x MES
Ligand PGE 3 x PGE
Ligand EDO 7 x EDO
Ligand PEG 7 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 87.389Å b: 63.245Å c: 96.733Å
α: 90° β: 93.21° γ: 90°
R-values:
R R work R free
0.168 0.165 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Controlling and enhancing CRISPR systems.
Shivram et al. (2021)
18 more