6anv

X-ray diffraction
2.26Å resolution

Crystal structure of anti-CRISPR protein AcrF1

Released:
Entry authors: Yang H, Patel DJ

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 49.77 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: L7P7M1 (Residues: 1-78; Coverage: 100%)
  • Canonical: P0AEY0 (Residues: 27-396; Coverage: 100%)
Gene names: ECs5017, JBD30_035, Z5632, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 87.389Å b: 63.245Å c: 96.733Å
α: 90° β: 93.21° γ: 90°
R-values:
R R work R free
0.168 0.165 0.223
Expression system: Escherichia coli BL21(DE3)