› 6ani

X-ray diffraction
2.4Å resolution

Coltuximab Fab in complex with anti-Kappa VHH domain

Released:
DOI: 10.2210/pdb6ani/pdb
PDB entry 6ani coloured by chain, front view.
PDB entry 6ani coloured by chain, side view.
PDB entry 6ani coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis of Enhanced Crystallizability Induced by a Molecular Chaperone for Antibody Antigen-Binding Fragments.
Ereño-Orbea J, Sicard T, Cui H, Carson J, Hermans P, Julien JP
J Mol Biol 430 322-336 (2018)
PMID: 29277294

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211741 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Anti-Kappa VHH domain Chains: A, K
Molecule details ›
Chains: A, K
Length: 121 amino acids
Theoretical weight: 10.32 KDa
Source organism: Lama glama
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
Coltuximab Fab heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 223 amino acids
Theoretical weight: 23.84 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Coltuximab Fab light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 211 amino acids
Theoretical weight: 23.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 182.39Å b: 48.301Å c: 128.547Å
α: 90° β: 104.07° γ: 90°
R-values:
R R work R free
0.247 0.245 0.286
Expression systems:
  • Escherichia coli-Pichia pastoris shuttle vector pPpARG4
  • Homo sapiens

Quick links

Citations

1 review citation

Exploring cellular biochemistry with nanobodies.
Cheloha et al. (2020)
22 other articles

2 mentions without citation

Mapping the influence of molecular structure on rates of electron transfer using direct measurements of the electron spin-spin exchange interaction.
Lukas et al. (2003)
1 more