› 6agm

X-ray diffraction
2Å resolution

Molecular basis for feedback inhibition of tyrosine-regulated 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from Escherichia coli

Released:
DOI: 10.2210/pdb6agm/pdb
PDB entry 6agm coloured by chain, front view.
PDB entry 6agm coloured by chain, side view.
PDB entry 6agm coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular basis for feedback inhibition of tyrosine-regulated 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from Escherichia coli.
Cui D, Deng A, Bai H, Yang Z, Liang Y, Liu Z, Qiu Q, Wang L, Liu S, Zhang Y, Shi Y, Qi J, Wen T
J Struct Biol 206 322-334 (2019)
PMID: 30946901

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 355 amino acids
Theoretical weight: 38.77 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00888 (Residues: 1-355; Coverage: 100%)
Gene names: JW2582, aroF, b2601
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand TYR 4 x TYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 54.189Å b: 133.859Å c: 87.67Å
α: 90° β: 91.23° γ: 90°
R-values:
R R work R free
0.195 0.193 0.229
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Biotechnological Production of the Sunscreen Pigment Scytonemin in Cyanobacteria: Progress and Strategy.
Gao et al. (2021)
3 other articles

1 mention without citation

Protein engineering for feedback resistance in 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.
Jayaraman et al. (2022)