X-ray diffraction
2.4Å resolution

Proton pyrophosphatase-E225S mutant

Source organism: Vigna radiata var. radiata
Entry authors: Tsai J-Y, Li K-M, Sun Y-J

Function and Biology Details

Reaction catalysed:
Diphosphate + H(2)O + H(+)(Side 1) = 2 phosphate + H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Pyrophosphate-energized vacuolar membrane proton pump Chains: A, B
Molecule details ›
Chains: A, B
Length: 766 amino acids
Theoretical weight: 80.05 KDa
Source organism: Vigna radiata var. radiata
Expression system: Saccharomyces cerevisiae
  • Canonical: P21616 (Residues: 1-766; Coverage: 100%)
Sequence domains: Inorganic H+ pyrophosphatase

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: C2
Unit cell:
a: 218.124Å b: 88.194Å c: 158.956Å
α: 90° β: 125.38° γ: 90°
R R work R free
0.192 0.191 0.226
Expression system: Saccharomyces cerevisiae