6af0

X-ray diffraction
2.88Å resolution

Structure of Ctr9, Paf1 and Cdc73 ternary complex from Myceliophthora thermophila

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ctr9 protein Chain: A
Molecule details ›
Chain: A
Length: 939 amino acids
Theoretical weight: 106.25 KDa
Source organism: Thermothelomyces thermophilus ATCC 42464
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G2QC65 (Residues: 30-967; Coverage: 71%)
Gene name: MYCTH_2305158
Sequence domains:
Paf1 protein Chain: P
Molecule details ›
Chain: P
Length: 121 amino acids
Theoretical weight: 12.98 KDa
Source organism: Thermothelomyces thermophilus ATCC 42464
Expression system: Escherichia coli
UniProt:
  • Canonical: G2QDK9 (Residues: 1-120; Coverage: 23%)
Gene name: MYCTH_2303763
CDC73_C domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 74 amino acids
Theoretical weight: 7.66 KDa
Source organism: Thermothelomyces thermophilus ATCC 42464
Expression system: Escherichia coli
UniProt:
  • Canonical: G2Q3X1 (Residues: 155-227; Coverage: 18%)
Gene name: MYCTH_2298931

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 99.867Å b: 237.789Å c: 62.737Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.309
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli