6acx

X-ray diffraction
3.5Å resolution

Crystal structure of Mycobacterium smegmatis Mfd in complex with ADP + Pi at 3.5 A resolution.

Released:
Entry authors: Putta S, Fox GC, Walsh MA, Rao DN, Nagaraja V, Natesh R

Function and Biology Details

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription-repair-coupling factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 1235 amino acids
Theoretical weight: 133.44 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0R3C5 (Residues: 1-1215; Coverage: 100%)
Gene names: MSMEG_5423, mfd
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR-H
Spacegroup: P212121
Unit cell:
a: 83.659Å b: 160.44Å c: 214.529Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.273
Expression system: Escherichia coli